畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (7): 1394-1404.doi: 10.11843/j.issn.0366-6964.2018.07.008

• 营养与饲料 • 上一篇    下一篇

精料类型对肉羊饲粮养分消化率及尿嘌呤衍生物排出量的影响

富丽霞1,2, 马涛1, 刁其玉1*, 成述儒2, 宋雅喆1   

  1. 1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点试验室, 北京 100081;
    2. 甘肃农业大学动物科学技术学院, 兰州 730070
  • 收稿日期:2017-12-11 出版日期:2018-07-23 发布日期:2018-07-23
  • 通讯作者: 刁其玉,研究员,博士生导师,E-mail:diaoqiyu@caas.cn
  • 作者简介:富丽霞(1992-),女,甘肃白银人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:fulixia0202@126.com
  • 基金资助:

    国家重点研发计划(2016YFE0109000);国家肉羊产业技术体系建设专项资金(CARS-38)

Effect of Different Concentrates on Nutrient Digestibility and Excretion of Purine Derivatives in Urine of Mutton Sheep

FU Li-xia1,2, MA Tao1, DIAO Qi-yu1*, CHENG Shu-ru2, SONG Ya-zhe1   

  1. 1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-12-11 Online:2018-07-23 Published:2018-07-23

摘要:

旨在建立利用嘌呤衍生物排出量估测微生物氮产量的模型。本研究选择10只平均体重为(47.4±4.4) kg健康的杜寒杂交成年公羊。10种全混颗粒饲粮,单一精料替换比例30%,每个处理10只羊,每个重复1只羊,试验按时间和饲粮划分为10期进行;每种饲粮饲喂20 d,其中预试期15 d,正试期5 d;整体试验持续200 d。试验测定了饲喂10种不同精料饲粮肉羊的营养物质消化率及尿嘌呤衍生物(PD)排出量。结果表明:1)饲粮营养成分的消化率与营养成分含量存在相关性,精料类型不同饲粮消化率有差异,饲粮可消化蛋白质(DCP)的最佳预测因子是粗蛋白质(CP);10种饲粮的摄入氮、尿氮、粪氮等均因精料类型不同呈极显著差异(P<0.01)。2)10种饲粮的尿囊素、尿酸、黄嘌呤(包括次黄嘌呤)占PD排出量的比例均因饲粮类型的不同呈极显著差异(P<0.01),占PD排出量的比例范围分别是85.60%~92.47%、2.54%~7.18%、3.20%~7.41%;10种饲粮的嘌呤氮指数(PNI)因饲粮类型的不同呈极显著差异(P<0.01),变化范围是0.03~0.10,然而PD排出量与摄入氮和微生物氮(MN)存在强的线性相关。摄入氮与氮表观消化率、尿氮和氮沉积具有强相关关系,建立的沉积氮(Y)估测方程式:Y=0.244 9×摄入氮-1.319 9(R2=0.825);PD排出量与摄入氮以及MN均存在线性强相关关系,建立的估测方程式分别是PD=0.119 7×摄入氮+10.161(R2=0.925)、MN=0.746 1×PD+1.785 4(R2=0.898)。

Abstract:

The aim of this study was to establish a model for estimating microbial nitrogen with purine derivatives. Ten Dorper×thin-tailed Han crossbred, non-castrated adult rams with(47.4±4.4) kg BW were assigned to 10 experimental diet treatments with different concentrate sources in a single factor design, and 10 rams in each treatment. The experimental diet included 10 pelleted TMR with a single concentrate ratio of 30%. The experiment included 10 periods in which 10 lambs were fed with one of the 10 pelleted TMR. Each period lasted for 20 d(15 d for adaptation and 5 d for trial period) and the total experiment lasted for 200 d. This experiment study the effect of 10 different concentrates on nutrient digestibility and excretion of purine derivatives in urine of mutton sheep. The results showed that:1)The digestibility of nutrients in diets was related to the content of nutrients, and the digestibility of diets with different concentrates was different. The best predictors of dietary digestible crude protein(DCP) was crude protein(CP). The urinary nitrogen, fecal nitrogen and intake nitrogen of lambs fed 10 diets with different concentrates showed significant difference(P<0.01). 2) The proportion of allantoin, uric acid, xanthine(included hypoxanthine) in total purine derivative(PD) excretion were significantly different(P<0.01) among 10 treatments, and the ratios were 85.60%-92.47%, 2.54%-7.18%, 3.20%-7.41%, respectively. Purine nitrogen indexes were significantly different(P<0.01) among 10 treatments, and range was 0.03-0.10. However, PD excretion had a strong linear relationship with intake nitrogen and microbial nitrogen(MN). Intake N had a strong relationship with apparent digestibility of N, urine nitrogen and retained N. A linear relationship was found between retained N(Y) and intake N:Y=0.244 9×intake N-1.319 9(R2=0.825). A linear relationship was found between PD excretion and intake N:PD excretion=0.119 7×intake N+10.161(R2=0.925), and a linear relationship was found between PD excretion and MN:MN=0.746 1×PD+1.785 4(R2=0.898).

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